trypsin digestion
英
美
网络 胰蛋白酶消化; 胰蛋白酵素水解; 胰蛋白酶水解
双语例句
- Mechanism and Kinetic Model for Limited Trypsin Digestion of Protein in Rice Dregs
胰蛋白酶有限酶解米渣蛋白的机理及动力学模型研究 - METHODS The peptide mapping was analyzed by trypsin digestion and RP-HPLC analysis.
方法采用胰蛋白酶裂解-反相高效液相色谱法测定肽图； - Influence on drug yields in rabbit liver and blood after trypsin digestion combined with EDTA
胰蛋白酶消化法合用乙二胺四醋酸二钠对兔肝和血中药物得率的影响 - Low concentration and shortened time of trypsin digestion and mechanical dissociation were adopted to conduct culture.
方法：采用低浓度、短时间胰酶消化和机械分离相结合的方法制备皮层神经元悬液，进行培养。 - To culture human conjunctiva fibroblast cells by trypsin digestion method and describe their growth features in vitro.
方法：1、用胰蛋白酶消化法取材人结膜成纤维细胞，并描述人结膜成纤维细胞的生物学特性。 - Eight sheep fetal fibroblast lines and four female adult Dorset ear fibroblast lines were established by trypsin digestion and tissue attached culture method respectively.
采用胰酶消化法和组织贴壁法分别建立了乙个绵羊胎儿成纤维细胞系sFFI－－8和4个雌性成年Dorset绵羊耳部成纤维细胞系DFI－4。 - Methods Human umbilical vein endothelial cells ( hUVEC) were cultured by trypsin digestion method.
采用酶消化法培养人脐静脉内皮细胞； - Then, using the improved explant and trypsin digestion methods, buffalo fetal fibroblast cells were efficiently isolated from the fetal's skin, and had been cultured in vitro for three generation.
然后，用经过改良的组织块法和胰酶消化法从水牛胎儿肌肉组织中成功分离得到水牛胎儿成纤维细胞，体外培养至第三代。 - T_1 is degraded gradually by trypsin digestion and simultaneously loses its capacity to combine with the corresponding receptor.
胸腺激素与受体的结合能力与它的生物活力是相关的，T1的活力随胰蛋白酶的消化而逐渐丧失，相应的T1与受体结合的能力亦逐渐降低至零。 - Peptide map was determined by trypsin digestion and followed RP-HPLC analysis.
胰酶消化及RP&HPLC法测定肽图； - Two kinds of pure cells were obtained by trypsin digestion and culture with serum containing and serum free medium.
二种方法均用胰酶不完全消化和加入血清或无血清培养相结合的方法得到纯化的内皮细胞。 - The relationship between the trypsin digestion of bone collagen and the component content of gelatin
胰蛋白酶降解骨胶原与明胶组份含量之间的关系 - The corresponding antigen was sensitive to trypsin digestion.
此抗体对E玫瑰花生成有明显阻断作用，其相应抗原又与E受体一样对胰酶消化敏感。 - Firstly, the collagen was obtained by the trypsin digestion and acid extraction from bovine tendon. Its purity and structure were characterized by Uv-Vis absorbance spectroscopy, Fourier transform infrared spectroscopy and amino acid analysis.
首先，采用胰酶消化和酸抽提相结合的方法提取了牛腱胶原，通过紫外-可见光谱、傅立叶变换红外光谱以及氨基酸分析表征了牛腱胶原的组成和结构。 - METHODS CFs were isolated by trypsin digestion method.
方法胰酶消化法分离、培养新生SD大鼠CFs，采用硝酸还原酶法和分光光度法分别测定基础状态和AVP干预下CFs的NOS活性和NO含量。 - METHODS Osteoblastic cells were isolated from newborn rat calvaria through trypsin digestion.
方法原代培养新生大鼠颅盖骨成骨细胞，3H胸腺嘧啶掺入法测定成骨细胞的增殖； - Methods CFs were isolated from neonatal SD rats by trypsin digestion and growth-arrested CFs were stimulated with Sim and/ or MVA at varied concentrations for different time lengths, and the DNA synthesis in the cells was measured by 3H-thymidine ( 3H-TdR) incorporation assay.
方法采用胰酶消化、差速贴壁法培养新生SD大鼠CFs，以3H-胸腺嘧啶核苷（3H-TdR）掺入法测定DNA合成，观察不同浓度Sim及MVA分别作用不同时间对CFsDNA合成功能的影响。 - Methods Human DAF was purified from human erythrocyte ghost by trypsin digestion, butanol extraction, sequential chromatography on DE32 and immunoaffinity chromatography.
方法经胰蛋白酶消化、正丁醇抽提及DE32离子交换色谱和抗体亲和层析等步骤从人红细胞膜影中分离纯化人红细胞膜DAF。 - Results: 1. Bone marrow culture-wide ( that is, law adherent) Isolation and Purification of goat MSCs by trypsin digestion for liquid and gradually purified after passage, method is simple and practical. 2.
结果：1.采用全骨髓培养法（即贴壁法）分离纯化骨髓间充质干细胞，经换液和胰酶消化传代后逐渐纯化，方法简单实用。 - CFs were isolated by trypsin digestion method from Wistar rats and identified by invert microscope and vimentin immunofluorescence. 2.
方法：1.采用胰酶消化法获得Wistar大鼠CFs。采用倒置相差显微镜和波形蛋白免疫荧光法鉴定细胞。 - We have modified the procedure to a sequential digestion of papain and DNase I for taking into account the fast traditional trypsin digestion.
考虑到传统的胰酶消化速度不好控制，我们用木瓜酶结合DNA酶I序贯消化。 - Dispase isolated enzymes and Trypsin digestion two-step method was used to prepare and cultivate normal human foreskin keratinocytes. 2.
方法：1.采用Dispase分离酶、胰蛋白酶两次消化法制备和培养正常人包皮组织角质形成细胞。 - Methods of trypsin digestion to separate MEF of ICR mouse which can be purified by subculture without using other methods and can used to prepare feeder layer. 2.
结论1.胰蛋白酶消化法分离ICR小鼠胚胎成纤维细胞，经传代培养即可纯化，无需其他的方法纯化MEF，可用于制备饲养层。 - The embryos were manually taken from the Nile Tilapia at somite differentiation stage. The ECs derived from the Nile Tilapia were isolated and sub-cultured with the trypsin digestion.
从发育至体节分化期尼罗罗非鱼手工分离出胚胎，采用胰蛋白酶消化法对尼罗罗非鱼ECs进行原代和继代分离培养。 - The medium was changed every two days time, until the cell grew to80% by trypsin digestion and passage.
每2天换液一次，细胞生长至80%时用胰蛋白酶进行消化传代。 - The ECs derived from the zebrafish at gastrula stage were isolated and sub-cultured with the trypsin digestion.
采用胰蛋白酶消化法从原肠期斑马鱼分离出ECs，并进行原代和继代培养。